TaqMan Search

All Gene Expression Assays

Search all TaqMan™ Assays for mRNA and long noncoding RNA analysis, including endogenous control and marker/reporter gene products.

Non-coding Only

TaqMan™ Noncoding RNA Assays can detect and quantify long (>200 bases) noncoding RNA (ncRNA) transcripts. Our collection covers long ncRNA transcripts from sources including NCBI RefSeq NR, RNAdb, and content on Invitrogen™ NCode™ Non-coding RNA Arrays.

Markers & Reporters

Marker & Reporter assays can be used to verify and quantify reporter gene products from expression vectors that have been introduced into cells or animals, such as lacZ or GFP.

Controls

Control Assays target commonly used endogenous control or reference RNAs. These Control Assays are typically used for normalization for relative gene expression quantitation data.

Fusion

TaqMan™ Gene Expression Fusion Assays are designed to detect fusion transcripts using real-time PCR. Our collection covers gene fusion transcripts from sources including NCBI Genbank and content on Oncomine™ products.

SNP Genotyping Assays

Search all TaqMan™ SNP Genotyping Assays, including Drug Metabolism Genotyping Assays, and assays that are functionally tested or validated.

Drug Metabolism Assays

These assays are specifically designed to detect polymorphisms in 221 genes that code for various drug metabolism enzymes and drug transporters. Assays are tested using gDNA from 45 individuals from four ethnicities. The minor allele frequency for each population is available, but rare and mutant alleles may not be present in the test DNA.

All Copy Number Assays

Search all TaqMan™ Copy Number Assays and required TaqMan™ Copy Number Reference Assays.

Markers & Reporters

Marker & Reporter assays are designed to detect the copy number of marker and reporter genes from expression vectors that have been introduced into cells or animals, such as the lacZ reporter gene or antibiotic genes.

Recommended Use: Cell culture RNAi experiments

Silencer^® Select siRNAs incorporate the latest improvements in siRNA design, off-target effect prediction algorithms, and chemical modifications to yield siRNA with unrivaled efficacy, potency, and specificity. They are recommended for in vitro RNAi experiments.

Recommended Use: Animal experiments

Ambion^® In Vivo siRNAs are designed using the Silencer^® Select algorithm and incorporate chemical modifications that help provide superior serum stability for in vivo delivery.

Recommended Use: Continuity in cell culture and animal experiments

Stealth RNAi siRNAs uses their proprietary chemical modifications for restricting off-target effects and their effective algorithm for providing efficient knockdown. They are recommended for completing in vitro RNAi studies that were started using Stealth siRNAs. For new studies, we recommend Ambion Silencer Select siRNAs.

Recommended Use: Continuity in cell culture experiments

Silencer^® siRNAs are designed using our first-generation algorithm and do not include any chemical modifications. They are recommended for completing in vitro RNAi studies that were started using Silencer^® siRNA. For new studies, we recommend Silencer^® Select siRNAs.

TaqMan™ Advanced miRNA Assays

TaqMan™ Advanced miRNA Assays, based on a universal RT approach, provide even greater sensitivity in biological fluids and proven TaqMan™ specificity.

TaqMan™ miRNA Assays

TaqMan™ MicroRNA Assays, based on the specific stem-loop primer approach, are the gold standard for sensitivity and specificity.

Controls

The miRNA control assays are unique to each assay chemistry type (universal RT or miRNA- specific stem loop RT) and cannot be used with the other assay chemistry.

Primary MicroRNA Assays

TaqMan™ Pri-miRNA Assays quantitate primary microRNA (pri-miRNA) transcripts, the initial RNA molecules in the microRNA (miRNA) biogenesis pathway.

Mimics

Small, synthetic, double-stranded RNA molecules designed to mimic endogenous mature miRNAs. They are supplied ready-to-use; they can be introduced into cells using transfection or electroporation. We recommend mirVana^™ miRNA Mimics, but we continue to offer Ambion^® Pre-miR miRNA Precursors for completion of studies that were started using these first-generation products.

Inhibitors

Small, synthetic, single-stranded RNA molecules designed to specifically bind to and inhibit mature miRNAs from functioning. We recommend mirVana™ miRNA inhibitors, but we continue to offer Ambion^® Anti-miR™ miRNA Inhibitors for completion of studies that were started using these first-generation products.

Mutation Detection: Digital PCR

Search all Mutation Detection Assays using Digital PCR TaqMan™ Technology.

Mutation Detection: Real-Time PCR

Search all Mutation Detection Assays using Real-Time PCR TaqMan™ Technology.

All Arrays

Search all array formats, from 96-well to OpenArray® format (3072 throughholes)

96-well standard

Sets of pre-selected TaqMan™ Gene Expression assays for specific pathways, biomarker sets, or disease states in 96-well plate format

96-well Fast

The same sets of pre-selected TaqMan™ Gene Expression assays as our 96-well standard arrays, but optimized for Fast PCR conditions. A Fast PCR-compatible thermal block is required.

384-well

Sets of pre-selected TaqMan™ Gene Expression assays for specific pathways, biomarker sets, or disease states in 384-well microfluidic card format. A TaqMan™ Array block, available for our 7900HT, Viia7™ and QuantStudio™ 12K Flex systems, is required for

OpenArray

Sets of pre-selected TaqMan™ Gene Expression assays for specific pathways, biomarker sets, or disease states in OpenArray™ format. A QuantStudio™ 12K Flex or NT Cycler system is required for these arrays.

All Arrays

Search all array formats, from 96-well to OpenArray® format (3072 throughholes)

96-well standard

Sets of pre-selected TaqMan™ MicroRNA assays for specific pathways, biomarker sets, or disease states in 96-well plate format

96-well Fast

The same sets of pre-selected TaqMan™ MicroRNA assays as our 96-well standard arrays, but optimized for Fast PCR conditions. A Fast PCR-compatible thermal block is required.

384-well

Sets of pre-selected TaqMan™ MicroRNA assays for specific pathways, biomarker sets, or disease states in 384-well microfluidic card format. A TaqMan™ Array block, available for our 7900HT, Viia7™ and QuantStudio™ 12K Flex systems, is required for

OpenArray

Sets of pre-selected TaqMan™ MicroRNA assays for specific pathways, biomarker sets, or disease states in OpenArray™ format. A QuantStudio™ 12K Flex or NT Cycler system is required for these arrays.

Supported Search Terms:

Assay ID : e.g, "Hs00153283_m1"
Entrez Gene ID: e.g., "10332"
Gene Symbol: e.g., "CALCA"
RefSeq Accession #: e.g., "NM_005795"
GenBank RNA: e.g., "AI219647"
Protein ID: e.g., "Q8IWW8"
RNAdb ID: e.g., "LIT1011"
RNAdb Gene Symbol: e.g., "Jpx"
MicroArray Probe ID*: e.g., "208478_s_at"

*If you enter a microarray probe ID, your search will return the 3' most assay(s) for the corresponding gene

Supported Search Terms:

Assay ID: e.g. "Hs00326060_CE"
SNP ID: e.g. "rs12096805"
Entrez Gene ID: e.g. "6262
Gene Symbol: e.g. "JAK2"
Gene Name: e.g. "Janus kinase 2"
RefSeq Accession #: e.g. "NM_007294"
GenBank mRNA: e.g. CR536579.1"
Protein ID: e.g. "CAA66975.1"
Cosmic ID: e.g. "121007"
Sequence

ONLY the species listed below are available for search by sequence. A single species from the list must be selected, and that species must match the species of the input sequence:

H. sapiens (Human)
M. musculus (Mouse)
R. norvegicus (Rat)
A. thaliana (Arabidopsis)
B. taurus (Cattle)
C. elegans (Nematode)
C. familiaris (Canine)
C. hircus (Goat)
C. griseus (Chinese hamster)
D. melanogaster (Fruit fly)
D. rerio (Zebrafish)
E. caballus (Horse)
G. gallus (Chicken)
G. max (Soybean)
H. glaber (Mole-rat)
M. mulatta (Rhesus)
M. fascicularis (Cyno)
O. aries (Sheep)
O. cuniculus (Rabbit)
O. sativa (Rice)
S. pombe (Fission yeast)
S. scrofa (Pig)
Z. mays (Maize)
Marker and Reporter sequences(e.g. GFP, FLAG, etc)

Based on NCBI Reference Sequence: NC_012920.1

Any software that reports genomic coordinates must include the genome reference version in the file header. To be compatible with Thermo Fisher Scientific software and content, the version number must be either GRCh38.p2 or hg19.
Example for VCF files:
##reference=GRCh38.p2
##contig= <ID=7,length=159345973,assembly=GRCh38.p2>

Example for BED files:
track name="CHP2_designed"
description="Amplicon_Insert_CGP2"
db=hg38
reference=GRCh38.p2
masked_reference=GRCh38.p2.mask1

Supported Search Terms:

You may conduct a batch search using the following types of search terms:

Assay ID: e.g, "Hs00153283_m1"
Entrez Gene ID: e.g., "10332"
Gene Symbol: e.g., "CALCA"
RefSeq Accession #: e.g., "NM_005795"
GenBank RNA: e.g., "AI219647"
Protein ID: e.g., "Q8IWW8"
RNAdb ID: e.g., "LIT1011"
RNAdb Gene Symbol: e.g., "Jpx"
MicroArray Probe ID*: e.g., "208478_s_at"

*If you enter a microarray probe ID, your search will return the 3' most assay(s) for the corresponding gene

Name Name The Sequence name must be Entered, upto 30 characters in length.	Sequence Sequence: Please enter your sequence in the 5' to 3' direction. Sequences must be composed solely of the nucleotides A, C, G, T. Please convert U's to T's and remove any spaces, tabs, or line breaks. Sequence: Sequences must be composed solely of the nucleotides A, C, G, and U.	X Remove All
		X Remove	Invalid Valid
		X Remove	Invalid Valid
		X Remove	Invalid Valid

Name
The Sequence name must be Entered, upto 30 characters in length.

Sequence:

Please enter your sequence in the 5' to 3' direction.

Sequences must be composed solely of the nucleotides A, C, G, T.

Please convert U's to T's and remove any spaces,
tabs, or line breaks.

Sequence:

Sequences must be composed solely of the nucleotides A, C, G, and U.

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Number	Position/Start	Position/Stop
	Based on NCBI Reference Sequence: NC_012920.1	Based on NCBI Reference Sequence: NC_012920.1